In response to complex issues such as global climate change, human health, water supply and environmental protection, and sustainable development etc., international cooperation in Lake Science is growing highlighted. The enhanced cooperation seems to be necessary to achieve the goal of ecological civilization construction in China. This paper uses bibliometric methods to analyze the articles published in scholarly journals during 2001-2020 through Web of Science (WOS), issued on the topic of international cooperation on Lake Science. In addition,7 representative lake research institutes worldwide are selected for comparative analysis on major contents of international cooperation, and the main directions and the trend of international cooperation in Lake Science are discussed. The results show that the node advantages in the international cooperation network of Lake Science are decreasing, and more international cooperatives from different countries(regions)tend to be active. On the other, the number of cooperative countries(regions)and the international cooperation rate of China are growing rapidly, which reflects the important position of China in international cooperative relations. In western countries, their research team are generally influential on international cooperation, accordingly, the Chinese research team need to improve its academic influence. The important issues of international cooperation on Lake Science are about human-lake environment coordination, lake-basin management, lake ecological degradation and restoration, and response to global climate change, etc. Finally, this paper puts forward the suggestions that China should strive continuously to strengthen international cooperation in Lake Science research in the future.